DNA

Description

DNA Marker Low is useful for determination of DNA fragments 50 bp to 1,000 bp in size. The DNA size marker consists of 10 double-stranded, blunt-end DNA fragments. The DNA bands close to each other, 550 bp and 500 bp, 120 bp and 100 bp, are for reference indicators. These DNA fragments were fixed amount in solution of DNA Marker Low, which makes it possible to estimate amount of DNA. The DNA Marker Low is supplied in a ready-to-use mixture containing 8 mM EDTA, 8 % glycerol, 0.015 % bromphenol blue and 10 mM Tris-HCl (pH8.0).

Description

DNA Marker High is useful for determination of DNA fragments 300 bp to 10,000 bp in size. The DNA size marker consists of 13 double-stranded, blunt-end DNA fragments. The DNA bands close to each other, 525 bp and 500 bp, are for reference indicators. These DNA fragments were fixed amount in solution of DNA Marker High, which makes it possible to estimate amount of DNA. The DNA Marker High is supplied in a ready-to-use mixture containing 8 mM EDTA, 8 % glycerol, 0.015 % bromphenol blue and 10 mM Tris-HCl (pH8.0)

Description

6 X DNA loading Dye is useful for loading DNA samples into wells on agrarose gel electrophoresis. It contains EDTA, glycerol, bromphenol blue and 10 mM Tris-HCl (pH8.0).

Description

E.coli harboring the plasmid encoding the gene of alkaline phosphatase from Shewanella sp. SIB1 (PAP).

Description

Taq DNA polymerase catalyzes 5'-3' synthesis of DNA. The enzyme has been proved not having the 3'-5' exonuclease activity. The enzyme has proven to have high amplification yield, be stable at high temparetaure.

Description

HotTaq DNA polymerase catalyzes 5'-3' synthesis of DNA. The enzyme has been proved not having the 3' - 5'exonuclease activity. Prior the first PCR step the HotTaq DNA polymerase should be activated by 15 minute incubation at 95-97°C.

Description

RedTaq DNA polymerase catalyzes 5'-3' synthesis of DNA. The enzyme has been proved not having the 3'-5' exonuclease activity. The enzyme has proven to have high amplification yield, be stable at high temparetaure. Added inert dye will not have any interference to the reaction. Visual confirmation that the enzyme has been added and proper component mixing of the reaction has occurred. Samples can be loaded directly onto an agarose gel for electrophoresis with no loading dye addition.

Description

LongTaq DNA polymerase catalyzes 5'-3' synthesis of DNA. The enzyme has been proved not having the 3'-5' exonuclease activity. The enzyme has proven to have high amplification yield, be stable at high temparetaure.

Description

In addition to 5'-3' DNA polymerase activity, it also possesses 3'-5' exonuclease (proofreading) activity. Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to ten fold more accurate than normal Taq DNA polymerase. Consequently, Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.

Description

The 2 x Taq PCR Mix with MgCl2 is a premixed solution containing everything needed for successful PCR reaction except specific primers and DNA template. The mix includes high-quality recombinant Taq DNA polymerase, nucleotides and magnesium in a PCR reaction buffer. For the reaction set-up add the PCR Mix (10 or 25 uL) to the primers, template and water for the total reaction volume of 20 or 50 uL.