Alkaline Phosphatase (PAP)

Catalog # D0009





Size:1000 Units
Price: USD $ 257.00
order now, ship in 5 days

* The price is valid only in USA. Please select country.

Contact Info
  • +1-909-264-1399
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
  • Specification

    Product Description

    E.coli harboring the plasmid encoding the gene of alkaline phosphatase from Shewanella sp. SIB1 (PAP).


    5 units/uL

    Quality Control Testing

    DNase: When 0.5 ug of lamda Hind III digest was incubated with 10 units of this enzyme in a 40 ul reaction mixture for 18 hours at 37°C no degradation of the DNA fragment is observed on agarose gel electrophoresis.
    Rnase: No RNase activity is observed by the use of RnaseAlert assay (Ambion). In this assay the reaction mixture containing the fluorescent-labeled RNA substrate was incubated with 10 units of this enzyme for 1 hours at 37°C.

    Supplied Product

    Kit Content
    1.10x Alkaline Phosphatase Reaction Buffer
    2.Dilution Buffer (1x Reaction Buffer)

    Storage Buffer

    10 mM Tris-HCl (pH 7.5), 0.025 mM ZnCl, 20.25 mM MgCl2, 50% glycerol.

    Storage Instruction

    Store at -20°C.


    Unit Definition
    One unit is defined as the amount required to hydrolyzed 1.0 umole p-nitrophenyl phosphate per 1 minute in glycine/NaOH buffer at pH10.5 and 37°C

  • Applications

    Enzyme Activity

Contact Info
  • +1-909-264-1399
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
4 Products to Compare
Remove All