GAPDH recombinant monoclonal antibody, clone 9B1
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More Files
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human GAPDH.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human GAPDH.
Theoretical MW (kDa)
Calculated MW: 36
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
Immunofluorescence(1:20-1:200)
Western Blot(1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: HepG2 whole cell lysate; Lane 2: U87 whole tissue lysate; Lane 3: JK whole cell lysate.Immunohistochemistry
Immunohistochemistry image of GAPDH recombinant monoclonal antibody, clone 9B1 diluted at 1:50 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of HepG2 Cells with GAPDH recombinant monoclonal antibody, clone 9B1 at 1:20, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing Hela cells stained with GAPDH recombinant monoclonal antibody, clone 9B1 (red line) at 1:50. -
Gene Info — GAPDH
Entrez GeneID
2597Protein Accession#
P04406Gene Name
GAPDH
Gene Alias
G3PD, GAPD, MGC88685
Gene Description
glyceraldehyde-3-phosphate dehydrogenase
Omim ID
138400Gene Ontology
HyperlinkGene Summary
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
Other Designations
OTTHUMP00000174431|OTTHUMP00000174432|aging-associated gene 9 protein|glyceraldehyde 3-phosphate dehydrogenase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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Disease
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