GAPDH monoclonal antibody, clone RM114
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A431 cells using GAPDH monoclonal antibody, clone RM114 (Cat # MAB12829) at 1:1000 dilution.
Immunocytochemistry
Immunocytochemistry staining of A431 cells with GAPDH monoclonal antibody, clone RM114 (Cat # MAB12829) (Red) at 1:200 dilution. Nuclear DNA was stained with DAPI (Blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human GAPDH.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to C-terminus region of human GAPDH.
Sequence
N/A
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry (1:200)
Immunohistochemistry (1:400-1:4000)
Immunoprecipitation (1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A431 cells using GAPDH monoclonal antibody, clone RM114 (Cat # MAB12829) at 1:1000 dilution.Immunohistochemistry
Immunocytochemistry
Immunocytochemistry staining of A431 cells with GAPDH monoclonal antibody, clone RM114 (Cat # MAB12829) (Red) at 1:200 dilution. Nuclear DNA was stained with DAPI (Blue).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — GAPDH
Entrez GeneID
2597Protein Accession#
P04406Gene Name
GAPDH
Gene Alias
G3PD, GAPD, MGC88685
Gene Description
glyceraldehyde-3-phosphate dehydrogenase
Omim ID
138400Gene Ontology
HyperlinkGene Summary
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
Other Designations
OTTHUMP00000174431|OTTHUMP00000174432|aging-associated gene 9 protein|glyceraldehyde 3-phosphate dehydrogenase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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Disease
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