CD44 recombinant monoclonal antibody, clone 19H12
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: A549 whole cell lysate; Lane 2: MCF-7 whole cell lysate; Lane 3: HepG2 whole cell lysate.
Immunohistochemistry
Immunohistochemistry image of CD44 recombinant monoclonal antibody, clone 19H12 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of Hela Cells with CD44 recombinant monoclonal antibody, clone 19H12 at 1:25, counter-stained with DAPI.
Flow Cytometry
Overlay Peak curve showing Hela cells stained with CD44 recombinant monoclonal antibody, clone 19H12 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD44.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CD44.
Theoretical MW (kDa)
Calculated MW: 81
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
Immunofluorescence(1:20-1:200)
Western Blot(1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: A549 whole cell lysate; Lane 2: MCF-7 whole cell lysate; Lane 3: HepG2 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of CD44 recombinant monoclonal antibody, clone 19H12 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of Hela Cells with CD44 recombinant monoclonal antibody, clone 19H12 at 1:25, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing Hela cells stained with CD44 recombinant monoclonal antibody, clone 19H12 (red line) at 1:50. -
Gene Info — CD44
Entrez GeneID
960Protein Accession#
P16070Gene Name
CD44
Gene Alias
CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
Gene Description
CD44 molecule (Indian blood group)
Omim ID
107269Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq
Other Designations
CD44 antigen|CD44 antigen (homing function and Indian blood group system)|CDW44 antigen|GP90 lymphocyte homing/adhesion receptor|Hermes antigen|antigen gp90 homing receptor|cell adhesion molecule|cell surface glycoprotein CD44|chondroitin sulfate proteogl
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Interactome
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Pathway
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Disease
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