CD44 recombinant monoclonal antibody, clone Hermes -3
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Flow Cytometry
Flow cytometric analysis of human lymphocytes with CD44 recombinant monoclonal antibody, clone Hermes -3 (Cat # RAB03284).
Human lymphocytes were stained with an isotype control (3.0- panel A) or the rmeric version of RAB03284 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD44.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against purified Hermes-1 antigen from KCA, a human B lymphoblastoid line that binds preferentially to mucosal HEV.
Reactivity
Human
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Blocking
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Blocking
Flow Cytometry
Flow cytometric analysis of human lymphocytes with CD44 recombinant monoclonal antibody, clone Hermes -3 (Cat # RAB03284).
Human lymphocytes were stained with an isotype control (3.0- panel A) or the rmeric version of RAB03284 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FACSCanto flow-cytometer. -
Gene Info — CD44
Entrez GeneID
960Gene Name
CD44
Gene Alias
CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
Gene Description
CD44 molecule (Indian blood group)
Omim ID
107269Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq
Other Designations
CD44 antigen|CD44 antigen (homing function and Indian blood group system)|CDW44 antigen|GP90 lymphocyte homing/adhesion receptor|Hermes antigen|antigen gp90 homing receptor|cell adhesion molecule|cell surface glycoprotein CD44|chondroitin sulfate proteogl
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Interactome
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Pathway
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Disease
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