YAP1 (phospho S127) recombinant monoclonal antibody, clone 3F3
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate (treated with Calyculin A 100 nM/60 mins) and Lane 2: HepG2 whole cell lysate (not treated) with YAP1 (phospho S127) recombinant monoclonal antibody, clone 3F3 (Cat # RAB04258).
Immunohistochemistry
Immunohistochemical staining of human endometrial cancer with YAP1 (phospho S127) recombinant monoclonal antibody, clone 3F3 (Cat # RAB04258) (diluated at 1:100).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human YAP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S127 of human YAP1.
Theoretical MW (kDa)
Calculated MW: 65 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate (treated with Calyculin A 100 nM/60 mins) and Lane 2: HepG2 whole cell lysate (not treated) with YAP1 (phospho S127) recombinant monoclonal antibody, clone 3F3 (Cat # RAB04258).Immunohistochemistry
Immunohistochemical staining of human endometrial cancer with YAP1 (phospho S127) recombinant monoclonal antibody, clone 3F3 (Cat # RAB04258) (diluated at 1:100).Enzyme-linked Immunoabsorbent Assay
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Gene Info — YAP1
Entrez GeneID
10413Protein Accession#
P46937Gene Name
YAP1
Gene Alias
YAP, YAP2, YAP65, YKI
Gene Description
Yes-associated protein 1, 65kDa
Omim ID
606608Gene Ontology
HyperlinkGene Summary
This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq
Other Designations
yes-associated protein 2
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Interactome
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Disease
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