YAP1 monoclonal antibody (M01J), clone 2F12
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant YAP1.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
YAP1 (NP_006097, 53 a.a. ~ 161 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QIVHVRGDSETDLEALFNAVMNPKTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLGAVSPGTLTPTGVVSGPAATPTAQHLR
Host
Mouse
Reactivity
Human, Mouse
Interspecies Antigen Sequence
Mouse (85)
Preparation Method
Cell Culture Production
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
YAP1 monoclonal antibody (M01J), clone 2F12. Western Blot analysis of YAP1 expression in NIH/3T3.Western Blot (Transfected lysate)
Western Blot analysis of YAP1 expression in transfected 293T cell line by YAP1 monoclonal antibody (M01J), clone 2F12.
Lane 1: YAP1 transfected lysate (Predicted MW: 54.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to YAP1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged YAP1 is 0.03 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to YAP1 on HeLa cell . [antibody concentration 30 ug/ml] -
Gene Info — YAP1
Entrez GeneID
10413GeneBank Accession#
NM_006106Protein Accession#
NP_006097Gene Name
YAP1
Gene Alias
YAP, YAP2, YAP65, YKI
Gene Description
Yes-associated protein 1, 65kDa
Omim ID
606608Gene Ontology
HyperlinkGene Summary
This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq
Other Designations
yes-associated protein 2
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Interactome
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Disease
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