ILK recombinant monoclonal antibody, clone 6A5
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: HL60 whole cell lysate; Lane 3: A549 whole cell lysate; Lane 4: Mouse kidney tissue.
Immunofluorescence
Immunofluorescence staining of MCF7 Cells with ILK recombinant monoclonal antibody, clone 6A5 at 1:50, counter-stained with DAPI.
Immunoprecipitation
Immunoprecipitating MCF7 in Hela whole cell lysate.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and mouse ILK.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human ILK.
Theoretical MW (kDa)
Calculated MW: 52, 4
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence(1:20-1:200)
Immunoprecipitation(1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: HL60 whole cell lysate; Lane 3: A549 whole cell lysate; Lane 4: Mouse kidney tissue.Immunofluorescence
Immunofluorescence staining of MCF7 Cells with ILK recombinant monoclonal antibody, clone 6A5 at 1:50, counter-stained with DAPI.Immunoprecipitation
Immunoprecipitating MCF7 in Hela whole cell lysate.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ILK
Entrez GeneID
3611Protein Accession#
Q13418Gene Name
ILK
Gene Alias
DKFZp686F1765, P59
Gene Description
integrin-linked kinase
Omim ID
602366Gene Ontology
HyperlinkGene Summary
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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