TOP2A recombinant monoclonal antibody, clone 5B10
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: Jurkat whole cell lysate; Lane 3: K562 whole cell lysate; Lane 4: NIH/3T3 whole cell lysate.
Immunohistochemistry
Immunohistochemistry image of TOP2A recombinant monoclonal antibody, clone 5B10 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system.
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and mouse TOP2A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human TOP2A.
Theoretical MW (kDa)
Calculated MW: 175,
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: Jurkat whole cell lysate; Lane 3: K562 whole cell lysate; Lane 4: NIH/3T3 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of TOP2A recombinant monoclonal antibody, clone 5B10 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — TOP2A
Entrez GeneID
7153Protein Accession#
P11388Gene Name
TOP2A
Gene Alias
TOP2, TP2A
Gene Description
topoisomerase (DNA) II alpha 170kDa
Omim ID
126430Gene Ontology
HyperlinkGene Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq
Other Designations
DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme|topoisomerase (DNA) II alpha (170kD)
-
Interactome
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com