TOP2A (phospho S1106) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated Jurkat cell lysates, Lane 2: Synthesized peptide treated Jurkat cell lysates reacted with TOP2A (phospho S1106) polyclonal antibody (Cat # PAB29634) at 1:500-1:3000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue reacted with TOP2A (phospho S1106) polyclonal antibody (Cat # PAB29634) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human TOP2A.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S1106 of human TOP2A.
Host
Rabbit
Theoretical MW (kDa)
174
Reactivity
Human, Mouse
Specificity
TOP2A (phospho S1106) polyclonal antibody detects endogenous levels of human TOP2A only when phosphorylated at serine 1106.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated Jurkat cell lysates, Lane 2: Synthesized peptide treated Jurkat cell lysates reacted with TOP2A (phospho S1106) polyclonal antibody (Cat # PAB29634) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue reacted with TOP2A (phospho S1106) polyclonal antibody (Cat # PAB29634) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide. -
Gene Info — TOP2A
Entrez GeneID
7153Protein Accession#
P11388Gene Name
TOP2A
Gene Alias
TOP2, TP2A
Gene Description
topoisomerase (DNA) II alpha 170kDa
Omim ID
126430Gene Ontology
HyperlinkGene Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq
Other Designations
DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme|topoisomerase (DNA) II alpha (170kD)
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Interactome
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Disease
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