HMGB1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:MCF-7 whole cell lysate Lane2:PC12 whole cell lysate Lane3:F9 whole cell lysate with HMGB1 recombinant monoclonal antibody (Cat # RAB02663) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using HMGB1 recombinant monoclonal antibody (Cat # RAB02663). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells using HMGB1 recombinant monoclonal antibody (Cat # RAB02663)(green). The nuclear counter stain is DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against HMGB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant HMGB1.
Theoretical MW (kDa)
25
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of HMGB1 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:10-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:MCF-7 whole cell lysate Lane2:PC12 whole cell lysate Lane3:F9 whole cell lysate with HMGB1 recombinant monoclonal antibody (Cat # RAB02663) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using HMGB1 recombinant monoclonal antibody (Cat # RAB02663). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells using HMGB1 recombinant monoclonal antibody (Cat # RAB02663)(green). The nuclear counter stain is DAPI (blue).Immunofluorescence
Flow Cytometry
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Gene Info — HMGB1
Entrez GeneID
3146Protein Accession#
P09429Gene Name
HMGB1
Gene Alias
DKFZp686A04236, HMG1, HMG3, SBP-1
Gene Description
high-mobility group box 1
Omim ID
163905Gene Ontology
HyperlinkOther Designations
Amphoterin|OTTHUMP00000018199|OTTHUMP00000190860|Sulfoglucuronyl carbohydrate binding protein|high mobility group box 1|high mobility group protein 1|high-mobility group (nonhistone chromosomal) protein 1
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Interactome
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Pathway
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Disease
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