HMGB1 recombinant monoclonal antibody, clone R07-2D8
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HMGB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human HMGB1.
Theoretical MW (kDa)
Calculated MW: 25 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of HMGB1 in K562, rat Brain, C6, 3T3, Hela lysates using HMGB1 antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using HMGB1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Gene Info — HMGB1
Entrez GeneID
3146Protein Accession#
P09429Gene Name
HMGB1
Gene Alias
DKFZp686A04236, HMG1, HMG3, SBP-1
Gene Description
high-mobility group box 1
Omim ID
163905Gene Ontology
HyperlinkOther Designations
Amphoterin|OTTHUMP00000018199|OTTHUMP00000190860|Sulfoglucuronyl carbohydrate binding protein|high mobility group box 1|high mobility group protein 1|high-mobility group (nonhistone chromosomal) protein 1
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Interactome
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Disease
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