MERTK recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of MCF7 (A), 293T (B) whole cell lysates with MERTK recombinant monoclonal antibody (Cat # RAB02599).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using MERTK recombinant monoclonal antibody (Cat # RAB02599). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.129). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MERTK.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human MERTK.
Theoretical MW (kDa)
210
Reactivity
Human
Specificity
Recognizes endogenous levels of MERTK protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MCF7 (A), 293T (B) whole cell lysates with MERTK recombinant monoclonal antibody (Cat # RAB02599).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using MERTK recombinant monoclonal antibody (Cat # RAB02599). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.129). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — MERTK
Entrez GeneID
10461Protein Accession#
Q12866Gene Name
MERTK
Gene Alias
MER, MGC133349, RP38, c-mer
Gene Description
c-mer proto-oncogene tyrosine kinase
Gene Ontology
HyperlinkGene Summary
This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). [provided by RefSeq
Other Designations
MER receptor tyrosine kinase|STK kinase
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Interactome
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Disease
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