GFAP recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of extracts of various cell lines, using GFAP recombinant monoclonal antibody (Cat # RAB02392) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of paraffin-embedded rat brain using GFAP recombinant monoclonal antibody (Cat # RAB02392) at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against recombinant GFAP.
Antibody Species
Rabbit
Theoretical MW (kDa)
50
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of GFAP and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)Storage Buffer
In PBS, pH 7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of extracts of various cell lines, using GFAP recombinant monoclonal antibody (Cat # RAB02392) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of paraffin-embedded rat brain using GFAP recombinant monoclonal antibody (Cat # RAB02392) at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Immunocytochemistry
Immunofluorescence
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Gene Info — GFAP
Entrez GeneID
2670Protein Accession#
P14136Gene Name
GFAP
Gene Alias
FLJ45472
Gene Description
glial fibrillary acidic protein
Gene Ontology
HyperlinkGene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
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Interactome
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Disease
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