GFAP recombinant monoclonal antibody, clone 6B12
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: MCF-7 whole cell lysate; Lane 2: Mouse Brain tissue lysate; Lane 3: Rat Brain tissue lysate.
Immunohistochemistry
Immunohistochemistry image of GFAP recombinant monoclonal antibody, clone 6B12 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of SH-SY5Y Cells with GFAP recombinant monoclonal antibody, clone 6B12 at 1:200, counter-stained with DAPI.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human, mouse and rat GFAP.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human GFAP.
Theoretical MW (kDa)
Calculated MW: 50
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
Immunofluorescence(1:20-1:200)
Western Blot(1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: MCF-7 whole cell lysate; Lane 2: Mouse Brain tissue lysate; Lane 3: Rat Brain tissue lysate.Immunohistochemistry
Immunohistochemistry image of GFAP recombinant monoclonal antibody, clone 6B12 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of SH-SY5Y Cells with GFAP recombinant monoclonal antibody, clone 6B12 at 1:200, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing Jurkat cells stained with GFAP recombinant monoclonal antibody, clone 6B12 (red line) at 1:50. -
Gene Info — GFAP
Entrez GeneID
2670Protein Accession#
P14136Gene Name
GFAP
Gene Alias
FLJ45472
Gene Description
glial fibrillary acidic protein
Gene Ontology
HyperlinkGene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
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Interactome
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Disease
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