NCK1 recombinant monoclonal antibody, clone R09-7F6
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: rat brain, Lane 3: C6, Lane 4: 3T3 and Lane 5: Hela lysates with NCK1 recombinant monoclonal antibody, clone R09-7F6 (Cat # RAB02076).
Immunocytochemistry
Immunocytochemical staining of Hela with NCK1 recombinant monoclonal antibody, clone R09-7F6 (Cat # RAB02076). (A) NCK1 (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NCK1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human NCK1.
Theoretical MW (kDa)
Calculated MW: 43 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: rat brain, Lane 3: C6, Lane 4: 3T3 and Lane 5: Hela lysates with NCK1 recombinant monoclonal antibody, clone R09-7F6 (Cat # RAB02076).Immunohistochemistry
Immunocytochemistry
Immunocytochemical staining of Hela with NCK1 recombinant monoclonal antibody, clone R09-7F6 (Cat # RAB02076). (A) NCK1 (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — NCK1
Entrez GeneID
4690Protein Accession#
P16333Gene Name
NCK1
Gene Alias
MGC12668, NCK, NCKalpha
Gene Description
NCK adaptor protein 1
Omim ID
600508Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. [provided by RefSeq
Other Designations
NCK tyrosine kinase|SH2/SH3 adaptor protein NCK-alpha|melanoma NCK protein|non-catalytic region of tyrosine kinase
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Interactome
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Pathway
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