SDHA recombinant monoclonal antibody, clone R09-5K6
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More Files
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Western Blot
Western blot analysis of Lane 1: K562, Lane 2: C6 and Lane 3: 3T3 lysates with SDHA recombinant monoclonal antibody, clone R09-5K6 (Cat # RAB01923).
Immunocytochemistry
Immunocytochemical staining of Hela with SDHA recombinant monoclonal antibody, clone R09-5K6 (Cat # RAB01923). (A) SDHA (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SDHA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human SDHA.
Theoretical MW (kDa)
Calculated MW: 73 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: K562, Lane 2: C6 and Lane 3: 3T3 lysates with SDHA recombinant monoclonal antibody, clone R09-5K6 (Cat # RAB01923).Immunohistochemistry
Immunocytochemistry
Immunocytochemical staining of Hela with SDHA recombinant monoclonal antibody, clone R09-5K6 (Cat # RAB01923). (A) SDHA (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — SDHA
Entrez GeneID
6389Protein Accession#
P31040Gene Name
SDHA
Gene Alias
FP, SDH2, SDHF
Gene Description
succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Gene Ontology
HyperlinkGene Summary
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. [provided by RefSeq
Other Designations
succinate dehydrogenase complex flavoprotein subunit|succinate dehydrogenase complex, subunit A, flavoprotein
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Metabolic pathways
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