CD52 recombinant monoclonal antibody, clone Campath-1H
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More Files
- More Functions
Immunohistochemistry
Immunohistochemistry analysis of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H was used to stain samples at a concentration of 5 ug/mL.
Immunohistochemistry
Immunohistochemistry analysis of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H was used to stain samples at a concentration of 5 ug/mL.
Immunohistochemistry
Immunohistochemistry analysis of formalin fixed human thyroid cancer slices were were stained at 5 ug/mL.
Immunohistochemistry
Immunohistochemistry analysis of formaldehyde fixed paraffin embedded rat epididymis tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H was used to stain samples at a concentration of 5 ug/mL.
Immunofluorescence
Immunofluorescence analysis of fixed rat thymus tissue stained with the chimeric human IgG1 version of Campath-1H at 5 ug/mL followed by a FITC conjugated secondary antibody, showing membrane and cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right; FITC, DAPI and merged channels.
Flow Cytometry
Flow-cytometry were stained with an isotype control at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
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Specification
Product Description
Rat recombinant monoclonal antibody raised against human CD52.
Antibody Species
Rat
Immunogen
Original antibody is raised against human lymphocytes.
Reactivity
Human
Specificity
Human CD52 antigen, also known as CAMPATH-1. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG2b, Kappa
Recommend Usage
Flow cytometry
Immunofluorescence
Immunohistochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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Applications
Immunohistochemistry
Immunohistochemistry analysis of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H was used to stain samples at a concentration of 5 ug/mL.Immunohistochemistry
Immunohistochemistry analysis of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H was used to stain samples at a concentration of 5 ug/mL.Immunohistochemistry
Immunohistochemistry analysis of formalin fixed human thyroid cancer slices were were stained at 5 ug/mL.Immunohistochemistry
Immunohistochemistry analysis of formaldehyde fixed paraffin embedded rat epididymis tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H was used to stain samples at a concentration of 5 ug/mL.Immunofluorescence
Immunofluorescence analysis of fixed rat thymus tissue stained with the chimeric human IgG1 version of Campath-1H at 5 ug/mL followed by a FITC conjugated secondary antibody, showing membrane and cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right; FITC, DAPI and merged channels.Flow Cytometry
Flow-cytometry were stained with an isotype control at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer. -
Gene Info — CD52
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Interactome
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