CD52 monoclonal antibody, clone HI186
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Specification
Product Description
Mouse monoclonal antibody raised against native CD52.
Immunogen
Human tonsil.
Host
Mouse
Theoretical MW (kDa)
21-28
Reactivity
Human
Specificity
This antibody reacts with CD52 (CAMPATH-1), a 21-28 KDa glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 KDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract.
Form
Liquid
Purification
Protein A Chromatography
Concentration
1 mg/mL
Purity
> 95% by SDS-PAGE
Isotype
IgG2b
Recommend Usage
Flow Cytometry (2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide).
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Flow Cytometry
Flow cytometry analysis of CD52 in patients suffering with Acute Lymphoblastic Leukemia (CD52 monoclonal antibody, clone HI186, Cat # MAB0944; detection by Goat anti-mouse IgG2b PE). A: CD52 positive cALL (gate leukemic cells). B: CD52 negative pro T ALL (gate leukemic cells). -
Gene Info — CD52
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Interactome
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