ERBB3 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specifications
Product Description
Rabbit polyclonal antibody raised against partial recombinant ERBB3.
Immunogen
Recombinant GST fusion protein corresponding to amino acids 1283-1323 of human ERBB3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:10000-1:50000)
WB (1:500- 1:2000)
Immunoprecipitation (5 ul)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Immunoprecipitation
Combined immunoprecipitation and immunoblot of a human cell lysate using ERBB3 polyclonal antibody (Cat # PAB9951).
Lysates were prepared from a human LNCap prostate cell line both with (+) treatment with Heregulin-beta1 for 15' at 100 ng/mL and without (-) the addition of Heregulin-beta1.
The combination of immunoprecipitation and immunoblotting was performing using 5 uL of the ERBB3 polyclonal antibody for immunoprecipitation followed by immunoblot detection using an anti-phosphotyrosine antibody.
Visualization occurred using an ECL system.
Film exposure approximately 1'.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — ERBB3
Entrez GeneID
2065Gene Name
ERBB3
Gene Alias
ErbB-3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3
Gene Description
v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq
Other Designations
erbB-3|lethal congenital contracture syndrome 2|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
-
Interactomes
-
Pathways
-
Diseases
-
Publication Reference
-
Coexpression patterns of EGFR, HER2, HER3 and HER4 in non-melanoma skin cancer.
Krahn G, Leiter U, Kaskel P, Udart M, Utikal J, Bezold G, Peter RU.
European Journal of Cancer 2001 Jan; 37(2):251.
-
Expression of the receptor tyrosine kinases, epidermal growth factor receptor, ErbB2, and ErbB3, in human ocular surface epithelia.
Liu Z, Carvajal M, Carraway CA, Carraway K, Pflugfelder SC.
Cornea 2001 Jan; 20(1):81.
Application:IF, WB-Ti, Human, Cornea, Conjunctiva tissues, Ocular.
-
Neuregulin-induced association of Sos Ras exchange protein with HER2(erbB2)/HER3(erbB3) receptor complexes in Schwann cells through a specific Grb2-HER2(erbB2) interaction.
Goodearl A, Viehover A, Vartanian T.
Developmental Neuroscience 2001 Jan; 23(1):25.
Application:IP, WB-Ce, Rat, Schwann cells.
-
Coexpression patterns of EGFR, HER2, HER3 and HER4 in non-melanoma skin cancer.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com