ERBB3 polyclonal antibody

Catalog # PAB9951

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Size:250 ug
Price: USD $ 747.00
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  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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Immunoprecipitation
Application

Immunoprecipitation

Combined immunoprecipitation and immunoblot of a human cell lysate using ERBB3 polyclonal antibody (Cat # PAB9951).
Lysates were prepared from a human LNCap prostate cell line both with (+) treatment with Heregulin-beta1 for 15' at 100 ng/mL and without (-) the addition of Heregulin-beta1.
The combination of immunoprecipitation and immunoblotting was performing using 5 uL of the ERBB3 polyclonal antibody for immunoprecipitation followed by immunoblot detection using an anti-phosphotyrosine antibody.
Visualization occurred using an ECL system.
Film exposure approximately 1'.

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against partial recombinant ERBB3.

    Immunogen

    Recombinant GST fusion protein corresponding to amino acids 1283-1323 of human ERBB3.

    Host

    Rabbit

    Reactivity

    Human, Mouse, Rat

    Form

    Liquid

    Quality Control Testing

    Antibody Reactive Against Recombinant Protein.

    Recommend Usage

    ELISA (1:10000-1:50000)
    WB (1:500- 1:2000)
    Immunoprecipitation (5 ul)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Immunoprecipitation

    Combined immunoprecipitation and immunoblot of a human cell lysate using ERBB3 polyclonal antibody (Cat # PAB9951).
    Lysates were prepared from a human LNCap prostate cell line both with (+) treatment with Heregulin-beta1 for 15' at 100 ng/mL and without (-) the addition of Heregulin-beta1.
    The combination of immunoprecipitation and immunoblotting was performing using 5 uL of the ERBB3 polyclonal antibody for immunoprecipitation followed by immunoblot detection using an anti-phosphotyrosine antibody.
    Visualization occurred using an ECL system.
    Film exposure approximately 1'.

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — ERBB3

    Entrez GeneID

    2065

    Gene Name

    ERBB3

    Gene Alias

    ErbB-3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3

    Gene Description

    v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

    Omim ID

    190151 607598

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq

    Other Designations

    erbB-3|lethal congenital contracture syndrome 2|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3

  • Interactomes
  • Pathways
  • Diseases
  • Publication Reference
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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