SARS-CoV-2 S polyclonal antibody

Catalog # PAB31725

Size

Price

Stock

Quantity

Size:100 ug
Price: USD $ 479.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western Blot analysis of Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells. Loading: 15 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 S polyclonal antibody (Cat # PAB31725) (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using SARS-CoV-2 S polyclonal antibody (Cat # PAB31725, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and air epithelium of COVID-19 patient lung, but not in non-COVID-19 patient lung.

Immunofluorescence
Application

Immunofluorescence

Immunofluorescent analysis of 4% paraformaldehydefixed 293T cells labeling SARS-CoV-2 (COVID-19) Spike with Cat # PAB31725 at 1 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

A direct ELISA was performed using antigen or control peptide as coating antigen and this antibody as the capture antibody (1 ug/mL). Secondary: Goat anti-rabbit igG HRP conjugate at 1:20000 dilution. Detection range is from 0.5 to 1000 ng/mL.

  • Specifications

    Product Description

    Rabbit Polyclonal antibody raised against synthetic peptide of SARS-CoV-2 S.SARS-CoV-2,Coronavirus,COVID-19,COVID19,Wuhan virus,Wuhanvirus

    Immunogen

    A synthetic peptide corresponding to 20 amino acids near the C-terminus of SARS-CoV-2 Spike glycoprotein. The immunogen is located within the last 50 amino acids of SARS-CoV-2 Spike protein.

    Host

    Rabbit

    Reactivity

    SARS-CoV-2

    Form

    Liquid

    Purification

    Peptide affinity purification

    Isotype

    IgG

    Recommend Usage

    ELISA
    Immunofluorescence (1 ug/mL)
    Immunohistochemistry (0.2 ug/mL)
    Western Blot (1 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.02% sodium azide).

    Storage Instruction

    Store at 4°C for up to one year.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Transfected lysate)

    Western Blot analysis of Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells. Loading: 15 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 S polyclonal antibody (Cat # PAB31725) (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using SARS-CoV-2 S polyclonal antibody (Cat # PAB31725, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and air epithelium of COVID-19 patient lung, but not in non-COVID-19 patient lung.

    Immunofluorescence

    Immunofluorescent analysis of 4% paraformaldehydefixed 293T cells labeling SARS-CoV-2 (COVID-19) Spike with Cat # PAB31725 at 1 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

    Enzyme-linked Immunoabsorbent Assay

    A direct ELISA was performed using antigen or control peptide as coating antigen and this antibody as the capture antibody (1 ug/mL). Secondary: Goat anti-rabbit igG HRP conjugate at 1:20000 dilution. Detection range is from 0.5 to 1000 ng/mL.
  • Interactomes
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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