IRF3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251MG sp cell lysates with IRF3 polyclonal antibody (Cat # PAB30721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with IRF3 polyclonal antibody (Cat # PAB30721) shows moderate cytoplasmic positivity.
Immunofluorescence
Immunofluorescent staining of A-431 cells with IRF3 polyclonal antibody (Cat # PAB30721) (Green) shows positivity in cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human IRF3.
Immunogen
Recombinant protein corresponding to human IRF3.
Sequence
VNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQ
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251MG sp cell lysates with IRF3 polyclonal antibody (Cat # PAB30721).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with IRF3 polyclonal antibody (Cat # PAB30721) shows moderate cytoplasmic positivity.Immunofluorescence
Immunofluorescent staining of A-431 cells with IRF3 polyclonal antibody (Cat # PAB30721) (Green) shows positivity in cytoplasm. -
Gene Info — IRF3
Entrez GeneID
3661Protein Accession#
Q14653Gene Name
IRF3
Gene Alias
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Gene Description
interferon regulatory factor 3
Omim ID
603734Gene Ontology
HyperlinkGene Summary
IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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