RIPK2 (phospho S176) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated 293 cell lysates, Lane 2: Synthesized peptide treated 293 cell lysates reacted with RIPK2 (phospho S176) polyclonal antibody (Cat # PAB29639) at 1:500-1:3000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human brain tissue reacted with RIPK2 (phospho S176) polyclonal antibody (Cat # PAB29639) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RIPK2.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S176 of human RIPK2.
Host
Rabbit
Theoretical MW (kDa)
70
Reactivity
Human, Mouse
Specificity
RIPK2 (phospho S176) polyclonal antibody detects endogenous levels of human RIPK2 only when phosphorylated at serine 176.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated 293 cell lysates, Lane 2: Synthesized peptide treated 293 cell lysates reacted with RIPK2 (phospho S176) polyclonal antibody (Cat # PAB29639) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human brain tissue reacted with RIPK2 (phospho S176) polyclonal antibody (Cat # PAB29639) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide. -
Gene Info — RIPK2
Entrez GeneID
8767Protein Accession#
O43353Gene Name
RIPK2
Gene Alias
CARD3, CARDIAK, CCK, GIG30, RICK, RIP2
Gene Description
receptor-interacting serine-threonine kinase 2
Omim ID
603455Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq
Other Designations
CARD-carrying kinase|CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase|growth-inhibiting gene 30|receptor interacting protein 2|receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP
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Interactomes
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Pathways
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Diseases
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