EIF4ENIF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with EIF4ENIF1 polyclonal antibody (Cat # PAB29372) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human pancreas with EIF4ENIF1 polyclonal antibody (Cat # PAB29372) shows strong cytoplasmic positivity in exocrine glandular cells at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 with EIF4ENIF1 polyclonal antibody (Cat # PAB29372) at 1-4 ug/mL concentration shows positivity in vesicles.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human EIF4ENIF1.
Immunogen
Recombinant protein corresponding to human EIF4ENIF1.
Sequence
FAPIPLEDHAENKVDILEMLQKAKVDLKPLLSSLSANKEKLKESSHSGVVLSVEEVEAGLKGLKVDQQVKNSTPFMAEHLEETLSAVTNNRQLKKDGDMTAFNKLVSTMKASGTLPSQPKVSRNLESHLMSPAEI
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with EIF4ENIF1 polyclonal antibody (Cat # PAB29372) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human pancreas with EIF4ENIF1 polyclonal antibody (Cat # PAB29372) shows strong cytoplasmic positivity in exocrine glandular cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with EIF4ENIF1 polyclonal antibody (Cat # PAB29372) at 1-4 ug/mL concentration shows positivity in vesicles. -
Gene Info — EIF4ENIF1
Entrez GeneID
56478Gene Name
EIF4ENIF1
Gene Alias
4E-T, Clast4, FLJ21601, FLJ26551
Gene Description
eukaryotic translation initiation factor 4E nuclear import factor 1
Omim ID
607445Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
2610509L04Rik|OTTHUMP00000063276|eIF4E-transporter
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Interactome
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Publication Reference
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Eif4enif1 haploinsufficiency disrupts oocyte mitochondrial dynamics and leads to subfertility.
Yuxi Ding, Zequn He, Yanwei Sha, Kehkooi Kee, Lin Li.
Development 2023 Dec; 150(23):dev202151.
Application：WB-Ce, Mouse, Oocyte.
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Eif4enif1 haploinsufficiency disrupts oocyte mitochondrial dynamics and leads to subfertility.
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