EIF4ENIF1 MaxPab mouse polyclonal antibody (B01)
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human EIF4ENIF1 protein.
Immunogen
EIF4ENIF1 (AAH33028.1, 1 a.a. ~ 985 a.a) full-length human protein.
Sequence
MDRRSMGETESGDAFLDLKKPPASKCPHRYTKEELLDIKELPHSKQRPSCLSEKYDSDGVWDPEKWHASLYPASGRSSPVESLKKELDTDRPSLVRRIVDPRERVKEDDLDVVLSPQRRSFGGGCHVTAAVSSRRSGSPLEKDSDGLRLLGGRRIGSGRIISARTFEKDHRLSDKDLRDLRDRDRERDFKDKRFRREFGDSKRVFGERRRNDSYTEEEPEWFSAGPTSQSETIELTGFDDKILEEDHKGRKRTRRRTASVKEGIVECNGGVAEEDEVEVILAQEPAADQEVPRDAVLPEQSPGDFDFNEFFNLDKVPCLASMIEDVLGEGSVSASRFSRWFSNPSRSGSRSSSLGSTPHEELERLAGLEQAILSPGQNSGNYFAPIPLEDHAENKVDILEMLQKAKVDLKPLLSSLSANKEKLKESSHSGVVLSVEEVEAGLKGLKVDQQVKNSTPFMAEHLEETLSAVTNNRQLKKDGDMTAFNKLVSTMKASGTLPSQPKVSRNLESHLMSPAEIPGQPVPKNILQELLGQPVQRPASSNLLSGLMGSLEPTTSLLGQRAPSPPLSQVFQTRAASADYLRPRIPSPIGFTPGPQQLLGDPFQGMRKPMSPITAQMSQLELQQAALEGLALPHDLAVQAANFYQPGFGKPQVDRTRDGFRNRQQRVTKSPAPVHRGNSSSPAPAASITSMLSPSFTPTSVIRKMYESKEKSKEEPASGKAALGDSKEDTQKASEENLLSSSSVPSADRDSSPTTNSKLSALQRSSCSTPLSQANRYTKEQDYRPKATGRKTPTLASPVPTTPFLRPVHQVPLVPHVPMVRPAHRLHPGLVQRMLAQGVHPQHLPSLLQTGVLPPGMDLSHLQGISGPILGQPFYPLPAASHPLLNPRPGTPLHLAMVQQQLQRSVLHPPGSGSHAAAVSVQTTPQNVPSRSGLPHMHSQLEHRPSQRSSSPVGLAKWFGSDVLQQPLPSMPAKVISVDELEYRQ
Host
Mouse
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note
For IHC and IF applications, antibody purification with Protein A will be needed prior to use.
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Applications
Western Blot (Cell lysate)
EIF4ENIF1 MaxPab polyclonal antibody. Western Blot analysis of EIF4ENIF1 expression in Hela S3 NE.Western Blot (Cell lysate)
EIF4ENIF1 MaxPab polyclonal antibody. Western Blot analysis of EIF4ENIF1 expression in Jurkat.Western Blot (Transfected lysate)
Western Blot analysis of EIF4ENIF1 expression in transfected 293T cell line (H00056478-T01) by EIF4ENIF1 MaxPab polyclonal antibody.
Lane 1: EIF4ENIF1 transfected lysate(108.35 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to EIF4ENIF1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — EIF4ENIF1
Entrez GeneID
56478GeneBank Accession#
BC033028.1Protein Accession#
AAH33028.1Gene Name
EIF4ENIF1
Gene Alias
4E-T, Clast4, FLJ21601, FLJ26551
Gene Description
eukaryotic translation initiation factor 4E nuclear import factor 1
Omim ID
607445Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
2610509L04Rik|OTTHUMP00000063276|eIF4E-transporter
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Interactome
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Publication Reference
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Nucleocytoplasmic transport of the RNA-binding protein CELF2 regulates neural stem cell fates.
Melissa J MacPherson, Sarah L Erickson, Drayden Kopp, Pengqiang Wen, Mohamad-Reza Aghanoori, Shreeya Kedia, Kaylan M L Burns, Antonio Vitobello, Frederic Tran Mau-Them, Quentin Thomas, Nina B Gold, William Brucker, Louise Amlie-Wolf, Karen W Gripp, Olaf Bodamer, Laurence Faivre, Mikko Muona, Lara Menzies, Julia Baptista, Katie Guegan, Alison Male, Xing-Chang Wei, Guiqiong He, Quan Long, A Micheil Innes, Guang Yang.
Cell Reports 2021 Jun; 35(10):109226.
Application:IF, WB-Ti, PLA-Ce, Human, Human cortex, Human cortical cells.
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Nucleocytoplasmic transport of the RNA-binding protein CELF2 regulates neural stem cell fates.
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