IL25 (Human) Recombinant Protein
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More Files
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Surface Plasmon Resonance
Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-25 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human IL25 (Q9H293-1, Tyr33-Gly177) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Tyr33-Gly177
Host
Human
Theoretical MW (kDa)
17.6
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The affinity constant of 0.81nM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-25 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200). -
Gene Info — IL25
Entrez GeneID
64806Protein Accession#
Q9H293-1Gene Name
IL25
Gene Alias
IL-17E, IL-25, IL17E
Gene Description
interleukin 25
Omim ID
605658Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that shares the sequence similarity with IL17. This cytokine can induce NF-kappaB activation, and stimulate the production of IL8. Both this cytokine and IL17B are ligands for the cytokine receptor IL17BR. Studies of the similar gene in mice suggested that this cytokine may be a proinflammatory cytokine favoring Th2-type immune response. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
interleukin 17E
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Interactome
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Pathway
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