IL25 (Human) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
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Specification
Product Description
Human IL25 (Q9H293, 33 a.a. - 177 a.a.) partial length recombinant protein His tag expressed in HEK293 expression system.
Sequence
YSHWPSCCPSKGQDTSEELLRWSTVPVPPLEPARPNRHPESCRASEDGPLNSRAISPWRYELDRDLNRLPQDLYHARCLCPHCVSLQTGSHMDPRGNSELLYHNQTVFYRRPCHGEKGTHKGYCLERRLYRVSLACVCVRPRVMG
Host
Human
Theoretical MW (kDa)
17.8
Form
Liquid
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
Measured by the binding ability in a functional ELISA with Human IL-17 RB.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In Phosphate-Buffer Saline pH 7.4 (20% glycerol)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — IL25
Entrez GeneID
64806Protein Accession#
Q9H293Gene Name
IL25
Gene Alias
IL-17E, IL-25, IL17E
Gene Description
interleukin 25
Omim ID
605658Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that shares the sequence similarity with IL17. This cytokine can induce NF-kappaB activation, and stimulate the production of IL8. Both this cytokine and IL17B are ligands for the cytokine receptor IL17BR. Studies of the similar gene in mice suggested that this cytokine may be a proinflammatory cytokine favoring Th2-type immune response. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
interleukin 17E
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Interactome
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Pathway
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