Products

Product Description

Human CD274 (Q9NZQ7-1, 19 a.a. - 226 a.a.) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro,PD-L1,PDL1

Sequence

FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVI

Host

Human

Theoretical MW (kDa)

24.93

Form

Liquid

Preparation Method

Mammalian cell (Expi293, high-yield transient HEK293) expression system

Purity

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Endotoxin Level

< 1 EU per 1 ug of protein (determined by LAL method)

Activity

The EC₅₀ was 4.8 ng/mL, messured by ELISA at 0.5 ug/mL.

Quality Control Testing

SEC-HPLC and Tris-Bis PAGE

SEC-HPLC

The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.

Tris-Bis PAGE

Human PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Recommend Usage

Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

In PBS pH 7.4

Storage Instruction

Store at -80°C for 12 Month.
Aliquot to avoid repeated freezing and thawing.

Note

Result of bioactivity analysis