Products

Product Description

Human CD274 (NP_054862.1, 19 a.a. - 239 a.a.) partial recombinant protein with Fc, 6x His tag at C-terminal expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro,PD-L1,PDL1

Host

Human

Form

Lyophilized

Preparation Method

Mammalian cell (HEK293) expression system

Purification

Ni-sepharose purification

Purity

> 95% by SDS-PAGE

Endotoxin Level

< 0.1 EU/ug of the protein by LAL method.

Activity

Measured by the binding ability in a functional ELISA. Immobilized Recombinant Human PD-1 at 5 ug/mL (100 uL/well) can bind CD274 (Human) Recombinant Protein (Cat # P6775) with a linear range of 0.5-2.2 ug/mL.

Quality Control Testing

SDS-PAGE Stained with Coomassie Blue

CD274 (Human) Recombinant Protein (Cat # P6775) was determined by SDS-PAGE with Coomassie Blue, showing a band at 70-75 kDa.

Recommend Usage

SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from PBS, pH 7.4.

Storage Instruction

Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.

Note

Result of activity analysis