Lif (Mouse) Recombinant Protein
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Specification
Product Description
Mouse LIF (P09056) recombinant protein expressed in Escherichia coli.
Sequence
MSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAF
Host
Escherichia coli
Theoretical MW (kDa)
20
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
> 95% RP-HPLCActivity
Activity of murine LIF was determined by the M1 cell differentiation assay which was found to be < 0.01 ng/mL, corresponding to a specific activity of 100,000,000 IU/mg._x005F_x005F_x005F_x000D__x005F_x000D_ A standard of 50 Units is defined as the concentration of mouse LIF in 1.0 mL of tissue culture medium that induces the differentiation of 50% of M1 colonies.
Storage Buffer
Lyophilized from 20mM Phosphate buffer pH-7.4 and 0.02% Tween-20
Storage Instruction
Lyophilized although stable at room temperature for 3 weeks, should be stored desiccated below -20°C. Upon reconstitution should be stored at 4°Cbetween 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Lif
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