Products

Product Description

Mouse Lif recombinant protein with polyhistidine tag at the N-terminus expressed in Escherichia coli.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins

Sequence

SPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAF with polyhistidine tag at the N-terminus.

Host

Escherichia coli

Form

Lyophilized

Preparation Method

Escherichia coli expression system

Purity

> 95% as determined by SDS-PAGE.

Endotoxin Level

< 0.1 EU per 1 ug of the protein by the LAL method.

Activity

Measured by the induction of IL-6 secretion in M1 cells. The ED₅₀ for this effect is < 0.5 ng/mL. The specific activity of recombinant mouse LIF is > 2 x 10⁶ IU/mg.

Quality Control Testing

SDS-PAGE Stained with Coomassie Blue.

SDS-PAGE analysis of Lif (Mouse) Recombinant Protein.

Recommend Usage

SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from a solution containing 1X PBS, pH 7.4. Reconstitute the lyophilized powder in ddH₂O to 100 ug/mL.

Storage Instruction

Lyophilized protein should be stored at -20°C. Protein aliquots should be stored at-20°C to -80°C. This product is stable for one year.
Avoid repeated freeze/thaw cycles.