Lif (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Lif (P09056, 24 a.a. - 203 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
SPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAF
Host
Escherichia coli
Theoretical MW (kDa)
19.9
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/ug of protein by gel clotting method
Activity
ED50 < 0.01 ng/mL, measured by a cell differentiation assay using M1 cells, corresponding to a specific activity of > 1.0 × 108 units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 50 mM Tris, 150 mM NaCl, pH 8.0. Reconstitute the lyophilized powder in ddH2O up to 100 ug/mL.
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Lif
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