Products

Product Description

Human/Mouse/Rat INHBA (P12643/P21274/P49001) recombinant protein expressed in E.Coli.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins

Sequence

MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

Host

Escherichia coli

Theoretical MW (kDa)

13.0/26.1

Form

Lyophilized

Preparation Method

This product is produced with no animal or human origin raw products. All processing and handling employs animal free equipment and animal free protocols.

Purity

>= 95%

Endotoxin Level

<= 1 EUs/ug (Kinetic LAL)

Activity

ED₅₀ <= 250 ng/mL
Alkaline phosphatase activity induced in ATDC-5 cells

The values provided above are minimum expected values to pass internal requirements.

Quality Control Testing

Reducing and Non-Reducing SDS PAGE

1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel, stained with Coomassie Blue. Human BMP-2 has predicted MW of 26.0 KDa as a homodimer (with each subunit having a MW of 13.0 kDa).

Conformation

Dimer

Storage Buffer

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).

Storage Instruction

Stored at -20°C prior to reconstitution for 1 year.
After reconstitution with sterile water at 0.1 mg/mL, store at 4°C for 1 month. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C for 3 months.
Aliquot to avoid repeated freezing and thawing.
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Note

Result of activity analysis