CD200 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD200 R1, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 0.77 ng/ml determined by ELISA.
SEC-HPLC
The purity of Human CD200 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD200 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specifications
Product Description
Human CD200 (P41217-1, Gln31-Gly232) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
Gln31-Gly232
Host
Human
Theoretical MW (kDa)
49.2
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Quality Control Testing
SEC-HPLC
SEC-HPLC
The purity of Human CD200 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD200 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SEC-HPLC
Tris-Bis PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from filtered solution in PBS, pH 7.4 (5% trehalose).
Storage Instruction
After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD200 R1, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 0.77 ng/ml determined by ELISA.Functional Study
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Gene Info — CD200
Entrez GeneID
4345Protein Accession#
P41217-1Gene Name
CD200
Gene Alias
MOX1, MOX2, MRC, OX-2
Gene Description
CD200 molecule
Omim ID
155970Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD200 antigen|MRC OX-2 antigen|OX-2 membrane glycoprotein|antigen identified by monoclonal antibody MRC OX-2
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Interactomes
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Diseases
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