SIRT4 monoclonal antibody, clone 6A12D7

Catalog # MAB17147

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Size:100 ug
Price: USD $ 531.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western Blot analysis of (1) HEK293 cells, (2) SIRT4-hIgGFc transfected HEK293 cell lysate.

Immunocytochemistry
Application

Immunocytochemistry

Immunocytochemical staining of HeLa cells with SIRT4 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of SIRT4 monoclonal antibody, clone 6A12D7.

Flow Cytometry
Application

Flow Cytometry

Flow cytometric analysis of HeLa cells with SIRT4 monoclonal antibody (green) and negative control (red).

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against recombinant human SIRT4.

    Immunogen

    Recombinant protein corresponding to amino acid 215-314 of human SIRT4 from E. coli.

    Host

    Mouse

    Theoretical MW (kDa)

    35.2kDa

    Reactivity

    Human

    Form

    Liquid

    Isotype

    IgG1

    Recommend Usage

    ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry
    Immunocytochemistry (1:200-1:1000)
    Flow Cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.05% sodium azide).

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Transfected lysate)

    Western Blot analysis of (1) HEK293 cells, (2) SIRT4-hIgGFc transfected HEK293 cell lysate.

    Immunocytochemistry

    Immunocytochemical staining of HeLa cells with SIRT4 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of SIRT4 monoclonal antibody, clone 6A12D7.

    Flow Cytometry

    Flow cytometric analysis of HeLa cells with SIRT4 monoclonal antibody (green) and negative control (red).
  • Gene Info — SIRT4

    Entrez GeneID

    23409

    Gene Name

    SIRT4

    Gene Alias

    MGC130046, MGC130047, MGC57437, SIR2L4

    Gene Description

    sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)

    Omim ID

    604482

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq

    Other Designations

    sir2-like 4|sirtuin 4|sirtuin type 4

  • Interactomes
  • Diseases
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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