CAMK2G monoclonal antibody, clone 8G10C1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: PC-12 cell lysate; Lane 2: Jurkat cell lysate; Lane 3: T47D cell lysate; Lane 4: HepG2 cell lysate with CAMK2G monoclonal antibody, clone 8G10C1 (Cat# MAB12257) at 1:500-1:2000 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CAMK2G (AA: 322-481)-hIgGFc transfected HEK293 cells] with CAMK2G monoclonal antibody, clone 8G10C1 (Cat# MAB12257) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human CAMK2G (AA: 322-481) recombinant protein (Expected MW is 44 kDa) with CAMK2G monoclonal antibody, clone 8G10C1 (Cat# MAB12257) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human prostate cancer tissues (A) and bladder cancer tissues (B) with CAMK2G monoclonal antibody, clone 8G10C1 (Cat# MAB12257) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CAMK2G monoclonal antibody, clone 8G10C1 (Cat# MAB12257) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of Jurkat cells with CAMK2G monoclonal antibody, clone 8G10C1 (Cat# MAB12257) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CAMK2G.
Immunogen
Recombinant protein corresponding to amino acids 322-481 of human CAMK2G.
Host
Mouse
Theoretical MW (kDa)
62.6
Reactivity
Human, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: PC-12 cell lysate; Lane 2: Jurkat cell lysate; Lane 3: T47D cell lysate; Lane 4: HepG2 cell lysate with CAMK2G monoclonal antibody, clone 8G10C1 (Cat# MAB12257) at 1:500-1:2000 dilution.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CAMK2G (AA: 322-481)-hIgGFc transfected HEK293 cells] with CAMK2G monoclonal antibody, clone 8G10C1 (Cat# MAB12257) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human CAMK2G (AA: 322-481) recombinant protein (Expected MW is 44 kDa) with CAMK2G monoclonal antibody, clone 8G10C1 (Cat# MAB12257) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human prostate cancer tissues (A) and bladder cancer tissues (B) with CAMK2G monoclonal antibody, clone 8G10C1 (Cat# MAB12257) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CAMK2G monoclonal antibody, clone 8G10C1 (Cat# MAB12257) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of Jurkat cells with CAMK2G monoclonal antibody, clone 8G10C1 (Cat# MAB12257) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — CAMK2G
Entrez GeneID
818Gene Name
CAMK2G
Gene Alias
CAMK, CAMK-II, CAMKG, FLJ16043, MGC26678
Gene Description
calcium/calmodulin-dependent protein kinase II gamma
Omim ID
602123Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Six alternatively spliced variants that encode six different isoforms have been characterized to date. Additional alternative splice variants that encode different isoforms have been described, but their full-length nature has not been determined. [provided by RefSeq
Other Designations
CaM kinase II|OTTHUMP00000019843|OTTHUMP00000019844|calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma
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Interactome
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Pathway
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Disease
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