TUBA1 monoclonal antibody (M01), clone 2E11
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Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant TUBA1.
Immunogen
TUBA1 (AAH09238, 1 a.a. ~ 448 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE
Host
Mouse
Reactivity
Human
Isotype
IgG2b kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (75.02 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
TUBA1 monoclonal antibody (M01), clone 2E11. Western Blot analysis of TUBA1 expression in HeLa.Western Blot (Cell lysate)
TUBA1 monoclonal antibody (M01), clone 2E11. Western Blot analysis of TUBA1 expression in different Cell lines and Human Tissue.Western Blot (Transfected lysate)
Western Blot analysis of TUBA1 expression in transfected 293T cell line by TUBA1 monoclonal antibody (M01), clone 2E11.
Lane 1: TUBA1 transfected lysate(49.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to TUBA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of TUBA1 over-expressed 293 cell line, cotransfected with TUBA1 Validated Chimera RNAi ( Cat # H00007277-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TUBA1 monoclonal antibody (M01), clone 2E11 (Cat # H00007277-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to TUBA1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — TUBA4A
Entrez GeneID
7277GeneBank Accession#
BC009238Protein Accession#
AAH09238Gene Name
TUBA4A
Gene Alias
FLJ30169, H2-ALPHA, TUBA1
Gene Description
tubulin, alpha 4a
Omim ID
191110Gene Ontology
HyperlinkGene Summary
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq
Other Designations
tubulin alpha-1 chain|tubulin, alpha 1 (testis specific)
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Interactomes
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