RNF20 recombinant monoclonal antibody, clone 2H12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Hela whole cell lysate, 293 whole cell lysate with RNF20 recombinant monoclonal antibody, clone 2H12 (Cat # RAB07509).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human testis tissue using RNF20 recombinant monoclonal antibody, clone 2H12 (Cat # RAB07509) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence
Immunofluorescent staining of Hela Cells with RNF20 recombinant monoclonal antibody, clone 2H12 (Cat # RAB07509). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human RNF20.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human RNF20.
Theoretical MW (kDa)
114
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Hela whole cell lysate, 293 whole cell lysate with RNF20 recombinant monoclonal antibody, clone 2H12 (Cat # RAB07509).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human testis tissue using RNF20 recombinant monoclonal antibody, clone 2H12 (Cat # RAB07509) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Immunofluorescence
Immunofluorescent staining of Hela Cells with RNF20 recombinant monoclonal antibody, clone 2H12 (Cat # RAB07509). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).Enzyme-linked Immunoabsorbent Assay
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Gene Info — RNF20
Entrez GeneID
56254Protein Accession#
Q5VTR2Gene Name
RNF20
Gene Alias
BRE1, BRE1A, FLJ11189, FLJ20382, KIAA2779, MGC129667, MGC129668, hBRE1
Gene Description
ring finger protein 20
Omim ID
607699Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3. [provided by RefSeq
Other Designations
BRE1 E3 ubiquitin ligase homolog|homolog of S. cerevisiae BRE1
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