RNF20 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:MCF7 whole cell lysate Lane3:Jurkat whole cell lysate with RNF20 recombinant monoclonal antibody (Cat # RAB02719) at 1:1000 dilution.
Flow Cytometry
Flow cytometric analysis of K562 cells with RNF20 recombinant monoclonal antibody (Cat # RAB02719) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against RNF20.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant RNF20.
Theoretical MW (kDa)
114
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of RNF20 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:MCF7 whole cell lysate Lane3:Jurkat whole cell lysate with RNF20 recombinant monoclonal antibody (Cat # RAB02719) at 1:1000 dilution.Immunocytochemistry
Flow Cytometry
Flow cytometric analysis of K562 cells with RNF20 recombinant monoclonal antibody (Cat # RAB02719) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. -
Gene Info — RNF20
Entrez GeneID
56254Protein Accession#
Q5VTR2Gene Name
RNF20
Gene Alias
BRE1, BRE1A, FLJ11189, FLJ20382, KIAA2779, MGC129667, MGC129668, hBRE1
Gene Description
ring finger protein 20
Omim ID
607699Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3. [provided by RefSeq
Other Designations
BRE1 E3 ubiquitin ligase homolog|homolog of S. cerevisiae BRE1
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