HDAC6 recombinant monoclonal antibody, clone 2H12
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate, Lane 2: MCK-7 whole cell lysate, Lane 3: HepG2 whole cell lysate, Lane 4: K562 whole cell lysate and Lane 5: Jurkat whole cell lysate with HDAC6 recombinant monoclonal antibody, clone 2H12 (Cat # RAB04308).
Immunohistochemistry
Immunohistochemical staining of human liver cancer with HDAC6 recombinant monoclonal antibody, clone 2H12 (Cat # RAB04308) (diluated at 1:112.5)
Immunoprecipitation
Immunoprecipitation analysis of HepG2 cell lysate with HDAC6 recombinant monoclonal antibody, clone 2H12 (Cat # RAB04308).
Lane 1: rabbit control IgG, Lane 2: RAB04308 precipitates and Lane 3: Input (HepG2 whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HDAC6.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HDAC6.
Theoretical MW (kDa)
Calculated MW: 132,
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate, Lane 2: MCK-7 whole cell lysate, Lane 3: HepG2 whole cell lysate, Lane 4: K562 whole cell lysate and Lane 5: Jurkat whole cell lysate with HDAC6 recombinant monoclonal antibody, clone 2H12 (Cat # RAB04308).Immunohistochemistry
Immunohistochemical staining of human liver cancer with HDAC6 recombinant monoclonal antibody, clone 2H12 (Cat # RAB04308) (diluated at 1:112.5)Immunoprecipitation
Immunoprecipitation analysis of HepG2 cell lysate with HDAC6 recombinant monoclonal antibody, clone 2H12 (Cat # RAB04308).
Lane 1: rabbit control IgG, Lane 2: RAB04308 precipitates and Lane 3: Input (HepG2 whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HDAC6
Entrez GeneID
10013Protein Accession#
Q9UBN7Gene Name
HDAC6
Gene Alias
FLJ16239, HD6, JM21
Gene Description
histone deacetylase 6
Omim ID
300272Gene Ontology
HyperlinkGene Summary
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq
Other Designations
OTTHUMP00000032398
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Interactome
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Disease
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