Cd27 recombinant monoclonal antibody, clone LG.3A10
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More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Cd27 recombinant monoclonal antibody, clone LG.3A10 (Cat # RAB03204).
Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of RAB03204 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03204- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Cd27 recombinant monoclonal antibody, clone LG.3A10 (Cat # RAB03204) to mouse CD27-Fc fusion protein ELISA Plate coated with mouse CD27-Fc fusion protein (Pr00160-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.1 ng/ml was performed using RAB03204. For detection- a 1:4000 dilution of HRP-loat anti-ribody (Bio-Rad) was used.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Cd27.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a mixture of two murine CD27 tag-expressing Armenian hamster fibroblast clones (AR0524 and AR0530).
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Cd27 recombinant monoclonal antibody, clone LG.3A10 (Cat # RAB03204).
Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of RAB03204 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03204- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Cd27 recombinant monoclonal antibody, clone LG.3A10 (Cat # RAB03204) to mouse CD27-Fc fusion protein ELISA Plate coated with mouse CD27-Fc fusion protein (Pr00160-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.1 ng/ml was performed using RAB03204. For detection- a 1:4000 dilution of HRP-loat anti-ribody (Bio-Rad) was used.Flow Cytometry
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Gene Info — Cd27
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