Cd27 recombinant monoclonal antibody, clone RM27-3E5
Size:200 ug
Price:
USD $
850.00
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Cd27.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse CD27-human IgG1 Fc fusion protein.
Reactivity
Mouse
Form
Liquid
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunoprecipitation
Neutralization
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA of anti-CD27 antibody on CD27-Fc fusion protein- with a CD70 (CD27-L) competition assay. Binding curves of the r chimeric version of the anti-CD27 (Interleukin-2 receptor subunit alpha) Cd27 recombinant monoclonal antibody, clone RM27-3E5 (Cat #RAB03205) (red line) and isotype control (anti-Fluorescein antibody 3.0 4-4-20 (enhanced); blue line) to an ELISA plate coated with CD27-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10 mg/mL to 0.17 ng/ml of 24-558 was used. CD70 (CD27-L) is the ligand for CD27. CD70 Fc-fusion protein was added to a 10mg/mL solution of RAB03205 starting at 10mg/mL- and then at 3-fold dilutions down to 0.17ng/mL (red line). A negative control assay using 4-1BB Fc-fusion protein was also performed (yellow line). Inhibition of the binding of RAB03205 to CD27 by CD70 may be observed between 10-000 ng/mL and 123 ng/mL of Pr00161-23.0- whereas Pr00233-1.9 (4-1BB) does not inhibit RAB03205 binding. For signal detection- a 1:4000 dilution of HRP-lnti-human IgG antibody was used.Flow Cytometry
Neutralization
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Gene Info — Cd27
Contact Info
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