Escherichia coli RuvA polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Detection of RuvA (22kD) protein by Western blotting using this antibody.
Lane1 : RuvA protein 0.8 ng.
Lane2 : E. coli AB1157 crude extract.
Lane3 : E. coli AB1157 LexA mutant crude extract.
Expression of RuvA is enhanced by LexA mutation.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant Escherichia coli RuvA.
Immunogen
Recombinant protein corresponding to full length Escherichia coli RuvA.
Host
Rabbit
Reactivity
Escherichia coli
Form
Liquid
Recommend Usage
Western Blot (1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Detection of RuvA (22kD) protein by Western blotting using this antibody.
Lane1 : RuvA protein 0.8 ng.
Lane2 : E. coli AB1157 crude extract.
Lane3 : E. coli AB1157 LexA mutant crude extract.
Expression of RuvA is enhanced by LexA mutation.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ruvA
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Publication Reference
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Processing the holliday junction in homologous recombination.
Shinagawa H, Iwasaki H.
Trends in Biochemical Sciences 1996 Mar; 21(3):107.
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Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration.
Iwasaki H, Takahagi M, Nakata A, Shinagawa H.
Genes & Development 1992 Nov; 6(11):2214.
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Processing the holliday junction in homologous recombination.
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