Cd97 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot using Cd97 polyclonal antibody (Cat # PAB10186) shows detection of bands corresponding to free Fc-Cd97- (5EGF) (lower arrowhead) and Fc-Cd97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells.
The left lane contains lysate from cells transfected with control DNA.
The right lane contains lysate from COS cells expressing Fc-Cd97- (5EGF).
No staining was noted from bone marrow lysates taken from Cd97 knockout mice.
The identity of the band at ~65 KDa appearing in all lanes is not known.
The formation of the Cd97 complex is currently under investigation.
Approximately 10 uL of lysate was used in each lane.
A 1:1,000 dilution of the primary antibody was used.
The image was processed using a 10-sec exposure.
Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant Cd97.
Immunogen
Recombinant protein corresponding to amino acids 1-512 of mouse Cd97.
Host
Rabbit
Reactivity
Mouse, Rat
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:1000-1:5000)
Immunofluorescence (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using Cd97 polyclonal antibody (Cat # PAB10186) shows detection of bands corresponding to free Fc-Cd97- (5EGF) (lower arrowhead) and Fc-Cd97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells.
The left lane contains lysate from cells transfected with control DNA.
The right lane contains lysate from COS cells expressing Fc-Cd97- (5EGF).
No staining was noted from bone marrow lysates taken from Cd97 knockout mice.
The identity of the band at ~65 KDa appearing in all lanes is not known.
The formation of the Cd97 complex is currently under investigation.
Approximately 10 uL of lysate was used in each lane.
A 1:1,000 dilution of the primary antibody was used.
The image was processed using a 10-sec exposure.
Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Cd97
Entrez GeneID
26364Protein Accession#
NP_036055;Q9Z0M6Gene Name
Cd97
Gene Alias
AA409984, TM7LN1
Gene Description
CD97 antigen
Gene Ontology
HyperlinkOther Designations
EGF-TM7 receptor|OTTMUSP00000025113
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Publication Reference
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CD97, an adhesion receptor on inflammatory cells, stimulates angiogenesis through binding integrin counterreceptors on endothelial cells.
Wang T, Ward Y, Tian L, Lake R, Guedez L, Stetler-Stevenson WG, Kelly K.
Blood 2005 Apr; 105(7):2836.
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Proteolytic cleavage of the EMR2 receptor requires both the extracellular stalk and the GPS motif.
Chang GW, Stacey M, Kwakkenbos MJ, Hamann J, Gordon S, Lin HH.
FEBS Letters 2003 Jul; 547(1-3):145.
Application：Flow Cyt, WB-Tr, Human, Mouse, CHO-K1, HEK 293T cells.
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CD97, but not its closely related EGF-TM7 family member EMR2, is expressed on gastric, pancreatic, and esophageal carcinomas.
Aust G, Steinert M, Schutz A, Boltze C, Wahlbuhl M, Hamann J, Wobus M.
American Journal of Clinical Pathology 2002 Nov; 118(5):699.
Application：IHC-Fr, Human, Gastric, Pancreatic, Esophageal tissues.
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CD97, an adhesion receptor on inflammatory cells, stimulates angiogenesis through binding integrin counterreceptors on endothelial cells.
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