EFEMP1 (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human EFEMP1, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 13.0 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human EFEMP1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human EFEMP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
-
Specification
Product Description
Human EFEMP1 (Q12805-1, Gln18-Phe493) partial recombinant protein with His tag at N-Terminus expressed in HEK293 cells.
Sequence
Gln18-Phe493
Host
Human
Theoretical MW (kDa)
53.9
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 13.0 ng/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human EFEMP1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human EFEMP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
-
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human EFEMP1, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 13.0 ng/mL determined by ELISA.Functional Study
SDS-PAGE
-
Gene Info — EFEMP1
Entrez GeneID
2202Protein Accession#
Q12805-1Gene Name
EFEMP1
Gene Alias
DHRD, DRAD, FBLN3, FBNL, FLJ35535, MGC111353, MLVT, MTLV, S1-5
Gene Description
EGF-containing fibulin-like extracellular matrix protein 1
Gene Ontology
HyperlinkGene Summary
This gene spans approximately 18 kb of genomic DNA and consists of 12 exons. Alternative splice patterns in the 5' UTR result in three transcript variants encoding the same extracellular matrix protein. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. [provided by RefSeq
Other Designations
fibrillin-like|fibulin 3
-
Interactome
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com