KLRB1 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD161, His Tag at 1 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 11.4 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Anti-CD161 Antibody, hFc tag captured on CM5 Chip via Anti-hFc IgG can bind Human CD161, His Tag with an affinity constant of 78.8 nM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human CD161 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD161 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%
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Specification
Product Description
Human KLRB1 (Q12918-1, Gln67-Ser225) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Gln67-Ser225
Host
Human
Theoretical MW (kDa)
19.6
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 11.4 ng/mL, messured by ELISA at 1 ug/mL. The affinity constant of 78.8 nM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD161 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD161 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD161, His Tag at 1 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 11.4 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Anti-CD161 Antibody, hFc tag captured on CM5 Chip via Anti-hFc IgG can bind Human CD161, His Tag with an affinity constant of 78.8 nM as determined in SPR assay (Biacore T200). -
Gene Info — KLRB1
Entrez GeneID
3820Protein Accession#
Q12918-1Gene Name
KLRB1
Gene Alias
CD161, CLEC5B, MGC138614, NKR, NKR-P1, NKR-P1A, NKRP1A, hNKR-P1A
Gene Description
killer cell lectin-like receptor subfamily B, member 1
Omim ID
602890Gene Ontology
HyperlinkGene Summary
Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. [provided by RefSeq
Other Designations
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Interactome
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Disease
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