AKT1 monoclonal antibody, clone C20-A
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: AKT1 recombinant protein and mouse brain tissue lysate (Lane 2: 20 ug, Lane 3: 100 ug and Lane 4: 200 ug) with AKT1 monoclonal antibody, clone C20-A (Cat # MAB15914).
Immunocytochemistry
Immunocytochemical staining of HEK293 cells with AKT1 monoclonal antibody, clone C20-A (Cat # MAB15914) at 1:300 dilution.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human AKT1.
Immunogen
A synthetic peptide corresponding to amino acids 462-478 at C-terminus of human AKT1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
EVAC purification
Isotype
IgG
Recommend Usage
ELISA (1:100000-1:200000)
Immunocytochemistry
Immunoprecipitation
Western Blot (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl buffer, pH 8.0 (10 mg/mL BSA, 0.05% Sodium Azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: AKT1 recombinant protein and mouse brain tissue lysate (Lane 2: 20 ug, Lane 3: 100 ug and Lane 4: 200 ug) with AKT1 monoclonal antibody, clone C20-A (Cat # MAB15914).Immunocytochemistry
Immunocytochemical staining of HEK293 cells with AKT1 monoclonal antibody, clone C20-A (Cat # MAB15914) at 1:300 dilution.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT1
Entrez GeneID
207Protein Accession#
P31749Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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Neuroprotective effects of the anticancer drug NVP-BEZ235 (dactolisib) on amyloid-β 1-42 induced neurotoxicity and memory impairment.
Bellozi PM, Lima IV, Dória JG, Vieira ÉL, Campos AC, Candelario-Jalil E, Reis HJ, Teixeira AL, Ribeiro FM, de Oliveira AC.
Scientific Reports 2016 May; 6:25226.
Application：WB-Ti, Mouse, Hippocampus.
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Neuroprotective effects of the anticancer drug NVP-BEZ235 (dactolisib) on amyloid-β 1-42 induced neurotoxicity and memory impairment.
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