AKT1 (phospho S473) recombinant monoclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of Lane1:NIH/3T3 whole cell lysate treated with PDGF Lane2:NIH/3T3 whole cell lysate with AKT1 (phospho S473) recombinant monoclonal antibody (Cat # RAB02743) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using AKT1 (phospho S473) recombinant monoclonal antibody (Cat # RAB02743). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells using AKT1 (phospho S473) recombinant monoclonal antibody (Cat # RAB02743)(green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against AKT1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant AKT1.
Theoretical MW (kDa)
60
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of AKT1 only when phosphorylated at Ser473. This antibody also recognizes AKT2 and AKT3 when phosphorylated at the corresponding residues.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:NIH/3T3 whole cell lysate treated with PDGF Lane2:NIH/3T3 whole cell lysate with AKT1 (phospho S473) recombinant monoclonal antibody (Cat # RAB02743) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using AKT1 (phospho S473) recombinant monoclonal antibody (Cat # RAB02743). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells using AKT1 (phospho S473) recombinant monoclonal antibody (Cat # RAB02743)(green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — AKT1
Entrez GeneID
207Protein Accession#
P31749;P31751;Q9Y243Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactomes
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Pathways
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Diseases
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