NPHS1 monoclonal antibody, clone G17-H
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human NPHS1.
Immunogen
A synthetic peptide corresponding to amino acids 53-101 at C-terminus of the first Ig-like domain of human NPHS1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
EVAC purification
Isotype
IgG
Recommend Usage
ELISA (1:20000-1:50000)
Immunoprecipitation
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl buffer, pH 8.0 (10 mg/mL BSA, 0.05% Sodium Azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western Blot analysis of NPHS1 recombinant protein (Lane 1: 50 ng, Lane 2: 100 ng and Lane 3: 200 ng) with NPHS1 monoclonal antibody, clone G17-H (Cat # MAB15906).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — NPHS1
Entrez GeneID
4868Protein Accession#
O60500Gene Name
NPHS1
Gene Alias
CNF, NPHN
Gene Description
nephrosis 1, congenital, Finnish type (nephrin)
Gene Ontology
HyperlinkGene Summary
Nephrin is a kidney glomerular filtration barrier protein that is an essential component of the interpodocyte-spanning slit diaphragm. Mutations in the nephrin gene are associated with congenital nephrotic syndrome (NPHS1; MIM 256300).[supplied by OMIM
Other Designations
nephrin|renal glomerulus-specific cell adhesion receptor
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Interactome
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Disease
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